Alkaline Protease
High-Activity Subtilisin for Detergent, Leather & Protein Processing
Alkaline protease enzyme delivers 100,000–200,000 U/g for dependable hydrolysis in pH 8.0–11.0 and 40–60 °C industrial workflows. Fermented from Bacillus licheniformis, supplied in 25 kg drums with global shipping for commercial producers.

Alkaline Protease Enzyme Powder
100,000 – 200,000 U/g
How Alkaline Protease Catalyses the Reaction

Activity vs. Temperature & pH
Reaction-rate curves for optimal temperature and pH — useful for process design and dosing.

Why Source From Us
- Worldwide shipping to 35+ countries
- ISO 9001, HALAL & KOSHER certified
- Food-grade & industrial-grade product lines
- Full batch traceability & technical data sheets

Alkaline Protease Specifications
Alkaline Protease Enzyme Powder — 100,000 – 200,000 U/g
Enzyme Name
Alkaline Protease (EC 3.4.21.62)
Activity
100,000 – 200,000 U/g
Optimal pH
8.0 – 11.0
Optimal Temperature
40°C – 60°C
Appearance
White to light tan powder
Source
Bacillus licheniformis
Shelf Life
12 months (sealed, cool, dry place)
Packaging
25 kg fiber drums / custom packaging
MOQ
25 kg
Certifications
ISO 9001, HALAL, KOSHER, Food Grade
Applications
Alkaline protease hydrolyses proteins into peptides and amino acids under alkaline pH, serving detergent, leather, cleaning, and protein-recovery lines requiring consistent enzyme stability.
Laundry Detergent
Supports protein-stain removal in powder and liquid detergent systems; compatible with anionic surfactants and dosed at 0.3–0.8% in typical powder formulations.
Learn more →Leather Dehairing
Replaces sodium sulfide during dehairing and bating at pH 8–11 and 30–40 °C, improving grain preservation and lowering chemical load in tannery workflows.
Learn more →Fish Meal & Protein Recovery
Processes fish offal and poultry byproducts to achieve 15–25% degree of hydrolysis for soluble protein streams in feed and aquaculture supply chains.
Learn more →Pharmaceutical Peptides
Casein, soy, and whey hydrolysis to generate precise molecular weight peptide fractions suitable for downstream processing and industrial peptide manufacture.
Learn more →Industrial Cleaning
CIP cleaning in dairy, brewing, and beverage plants to remove protein fouling from stainless steel lines, tanks, and heat exchangers in alkaline conditions.
Learn more →Wheat Gluten Modification
Partial hydrolysis improves gluten solubility and functional properties for ingredient production with controlled alkaline wet-processing.
Learn more →Why Source From Us
Alkaline pH Stability for Industrial Lines
This alkaline protease maintains 100,000–200,000 U/g activity in pH 8–11 and 40–60 °C processes. Surfactant-compatibility data available for detergent formulation teams.
Complete Documentation for Procurement
Each shipment includes COA and TDS, plus ISO 9001, HALAL, KOSHER, and Food Grade certificates. RFQs typically returned within one business day.
Consistent Subtilisin Profile, Direct Supply
Bacillus licheniformis fermentation optimized for uniform subtilisins. Factory-direct purchase removes middle-man costs for bulk industrial orders.
Flexible Packaging, Global Delivery
Available in 25 kg drums or custom packs with 12-month shelf life. Shipments reach 80+ countries, supporting trials and scheduled replenishments.
Frequently Asked Questions
What is alkaline protease?
Alkaline protease is an industrial enzyme that cleaves peptide bonds under alkaline conditions. It is a subtilisin-type serine endopeptidase produced via Bacillus fermentation for detergent, leather, cleaning, and protein-processing applications.
What is the EC number of alkaline protease and which family does it belong to?
EC 3.4.21.62, belonging to the serine endopeptidase class and commonly identified as a subtilisin-type alkaline serine protease. This classification helps compare pH stability and compatibility between suppliers.
What is alkaline protease used for?
Applications include laundry detergent, leather dehairing, fish meal protein recovery, peptide production, CIP cleaning, and wheat gluten modification. One enzyme grade can be used across multiple alkaline process lines.
Is alkaline protease the same as Alcalase or subtilisin?
Alcalase is a trade name for a subtilisin-type alkaline protease. The product matches the EC 3.4.21.62 subtilisin profile used in alkaline industrial processing.
What dosage of alkaline protease is typical in laundry detergent formulations?
Typical dosage is 0.3–0.8% w/w in powder detergents and 0.3–1.2% w/w in liquid detergents based on active enzyme. Final doses depend on surfactant system, soil load, pH, and temperature; trials are recommended.
What are the optimal pH and temperature ranges for alkaline protease?
Optimal operation is pH 8.0–11.0 and 40–60 °C. This suits detergent, leather, and CIP processes requiring alkaline conditions. Performance may drop outside these ranges.
Is alkaline protease food grade? What certifications ship with every order?
Food-grade variants are available. Orders can include ISO 9001, HALAL, KOSHER, and Food Grade documentation. COA and TDS provided; MSDS available on request.
Can alkaline protease replace sodium sulfide in leather dehairing?
Yes, under pH 8–11 and controlled temperature, alkaline protease can reduce or replace sodium sulfide, improving hair release and grain preservation while lowering sulfide load.
Get a Free Sample & Quote
Specify your application and process parameters. We'll recommend an activity grade, send a free 100 g sample with COA/TDS, and quote bulk pricing within 24 hours.
Response
Response within 24 hours
Shipping
Ships from China to 80+ countries