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EC 3.4.21.62 · Bacillus licheniformis · Food Grade

Alkaline Protease
High-Activity Subtilisin for Detergent, Leather & Protein Processing

Alkaline protease enzyme delivers 100,000–200,000 U/g for dependable hydrolysis in pH 8.0–11.0 and 40–60 °C industrial workflows. Fermented from Bacillus licheniformis, supplied in 25 kg drums with global shipping for commercial producers.

15+Years experience
80+Countries shipped
ISO9001 certified
Alkaline Protease Enzyme Powder

Alkaline Protease Enzyme Powder

100,000 – 200,000 U/g

200,000 U/g
U/g Activity
Industrial-grade concentration
25 kg
Minimum Order
Sample + bulk available
35+
Countries
Global shipping network
ISO 9001
Certified
HALAL · KOSHER · Food Grade
How It Works

How Alkaline Protease Catalyses the Reaction

Alkaline Protease reaction mechanism — sucrose hydrolysis pathway in four steps
Operating Window

Activity vs. Temperature & pH

Reaction-rate curves for optimal temperature and pH — useful for process design and dosing.

Alkaline Protease reaction rate vs temperature and pH — bell curves with optimal zones shaded
Trust Signals

Why Source From Us

  • Worldwide shipping to 35+ countries
  • ISO 9001, HALAL & KOSHER certified
  • Food-grade & industrial-grade product lines
  • Full batch traceability & technical data sheets
Alkaline Protease quality & certifications badge — ISO 9001, HALAL, KOSHER, Food Grade
ISO 9001HALALKOSHERFood GradeNon-GMO OptionsFree Samples

Alkaline Protease Specifications

Alkaline Protease Enzyme Powder — 100,000 – 200,000 U/g

Enzyme Name

Alkaline Protease (EC 3.4.21.62)

Activity

100,000 – 200,000 U/g

Optimal pH

8.0 – 11.0

Optimal Temperature

40°C – 60°C

Appearance

White to light tan powder

Source

Bacillus licheniformis

Shelf Life

12 months (sealed, cool, dry place)

Packaging

25 kg fiber drums / custom packaging

MOQ

25 kg

Certifications

ISO 9001, HALAL, KOSHER, Food Grade

Applications

Alkaline protease hydrolyses proteins into peptides and amino acids under alkaline pH, serving detergent, leather, cleaning, and protein-recovery lines requiring consistent enzyme stability.

🧺

Laundry Detergent

Supports protein-stain removal in powder and liquid detergent systems; compatible with anionic surfactants and dosed at 0.3–0.8% in typical powder formulations.

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🧴

Leather Dehairing

Replaces sodium sulfide during dehairing and bating at pH 8–11 and 30–40 °C, improving grain preservation and lowering chemical load in tannery workflows.

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🐟

Fish Meal & Protein Recovery

Processes fish offal and poultry byproducts to achieve 15–25% degree of hydrolysis for soluble protein streams in feed and aquaculture supply chains.

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🧪

Pharmaceutical Peptides

Casein, soy, and whey hydrolysis to generate precise molecular weight peptide fractions suitable for downstream processing and industrial peptide manufacture.

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🧹

Industrial Cleaning

CIP cleaning in dairy, brewing, and beverage plants to remove protein fouling from stainless steel lines, tanks, and heat exchangers in alkaline conditions.

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🌾

Wheat Gluten Modification

Partial hydrolysis improves gluten solubility and functional properties for ingredient production with controlled alkaline wet-processing.

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Why Source From Us

Alkaline pH Stability for Industrial Lines

This alkaline protease maintains 100,000–200,000 U/g activity in pH 8–11 and 40–60 °C processes. Surfactant-compatibility data available for detergent formulation teams.

Complete Documentation for Procurement

Each shipment includes COA and TDS, plus ISO 9001, HALAL, KOSHER, and Food Grade certificates. RFQs typically returned within one business day.

Consistent Subtilisin Profile, Direct Supply

Bacillus licheniformis fermentation optimized for uniform subtilisins. Factory-direct purchase removes middle-man costs for bulk industrial orders.

Flexible Packaging, Global Delivery

Available in 25 kg drums or custom packs with 12-month shelf life. Shipments reach 80+ countries, supporting trials and scheduled replenishments.

Frequently Asked Questions

What is alkaline protease?

Alkaline protease is an industrial enzyme that cleaves peptide bonds under alkaline conditions. It is a subtilisin-type serine endopeptidase produced via Bacillus fermentation for detergent, leather, cleaning, and protein-processing applications.

What is the EC number of alkaline protease and which family does it belong to?

EC 3.4.21.62, belonging to the serine endopeptidase class and commonly identified as a subtilisin-type alkaline serine protease. This classification helps compare pH stability and compatibility between suppliers.

What is alkaline protease used for?

Applications include laundry detergent, leather dehairing, fish meal protein recovery, peptide production, CIP cleaning, and wheat gluten modification. One enzyme grade can be used across multiple alkaline process lines.

Is alkaline protease the same as Alcalase or subtilisin?

Alcalase is a trade name for a subtilisin-type alkaline protease. The product matches the EC 3.4.21.62 subtilisin profile used in alkaline industrial processing.

What dosage of alkaline protease is typical in laundry detergent formulations?

Typical dosage is 0.3–0.8% w/w in powder detergents and 0.3–1.2% w/w in liquid detergents based on active enzyme. Final doses depend on surfactant system, soil load, pH, and temperature; trials are recommended.

What are the optimal pH and temperature ranges for alkaline protease?

Optimal operation is pH 8.0–11.0 and 40–60 °C. This suits detergent, leather, and CIP processes requiring alkaline conditions. Performance may drop outside these ranges.

Is alkaline protease food grade? What certifications ship with every order?

Food-grade variants are available. Orders can include ISO 9001, HALAL, KOSHER, and Food Grade documentation. COA and TDS provided; MSDS available on request.

Can alkaline protease replace sodium sulfide in leather dehairing?

Yes, under pH 8–11 and controlled temperature, alkaline protease can reduce or replace sodium sulfide, improving hair release and grain preservation while lowering sulfide load.

Get a Free Sample & Quote

Specify your application and process parameters. We'll recommend an activity grade, send a free 100 g sample with COA/TDS, and quote bulk pricing within 24 hours.

Response

Response within 24 hours

Shipping

Ships from China to 80+ countries

Free sample included with every qualified inquiry